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human breast epithelial cell culture serum  (Celprogen Inc)


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    Celprogen Inc human breast epithelial cell culture serum
    Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b <t>Human</t> (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) <t>breast</t> cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and <t>cell</t> number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The <t>culture</t> media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks
    Human Breast Epithelial Cell Culture Serum, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+epithelial+cell+culture+serum+free+media/Human+Breast+Epithelial+Cell+Culture+Serum+Free+Media/pmc07376160-186-13-5
    Average 90 stars, based on 2 article reviews
    human breast epithelial cell culture serum - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "An enhanced chemopreventive effect of methyl donor S-adenosylmethionine in combination with 25-hydroxyvitamin D in blocking mammary tumor growth and metastasis"

    Article Title: An enhanced chemopreventive effect of methyl donor S-adenosylmethionine in combination with 25-hydroxyvitamin D in blocking mammary tumor growth and metastasis

    Journal: Bone Research

    doi: 10.1038/s41413-020-0103-6

    Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b Human (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) breast cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and cell number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The culture media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks
    Figure Legend Snippet: Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b Human (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) breast cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and cell number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The culture media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks

    Techniques Used: In Vitro, Clonogenic Cell Survival Assay, Staining, Microscopy

    Related Articles

    Cell Culture:

    Article Title: Methyl donor S-adenosylmethionine (SAM) supplementation attenuates breast cancer growth, invasion, and metastasis in vivo ; therapeutic and chemopreventive applications
    Article Snippet: .. The human breast epithelial cells (HBEC) were purchased from Celprogen (Cat# 36056-01) and were maintained in commercially available human breast epithelial cell culture serum free media (Celprogen, Cat# M36056-01). .. Cells were treated with SAM (New England Biolabs, Mississauga, Ontario, Canada; Catalog # B9003S) by directly adding it to regular growth medium under sterile conditions following the treatment plan shown in Figure .



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    human breast epithelial cell culture serum  (Celprogen Inc)
    90
    Celprogen Inc human breast epithelial cell culture serum
    Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b <t>Human</t> (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) <t>breast</t> cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and <t>cell</t> number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The <t>culture</t> media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks
    Human Breast Epithelial Cell Culture Serum, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+epithelial+cell+culture+serum+free+media/Human+Breast+Epithelial+Cell+Culture+Serum+Free+Media/pmc07376160-186-13-5
    Average 90 stars, based on 1 article reviews
    human breast epithelial cell culture serum - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    human breast epithelial cell culture serum free media  (Celprogen Inc)
    90
    Celprogen Inc human breast epithelial cell culture serum free media
    Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b <t>Human</t> (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) <t>breast</t> cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and <t>cell</t> number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The <t>culture</t> media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks
    Human Breast Epithelial Cell Culture Serum Free Media, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+epithelial+cell+culture+serum+free+media/Human+Breast+Epithelial+Cell+Culture+Serum+Free+Media/pmc05797041-181-18-26
    Average 90 stars, based on 1 article reviews
    human breast epithelial cell culture serum free media - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b Human (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) breast cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and cell number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The culture media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks

    Journal: Bone Research

    Article Title: An enhanced chemopreventive effect of methyl donor S-adenosylmethionine in combination with 25-hydroxyvitamin D in blocking mammary tumor growth and metastasis

    doi: 10.1038/s41413-020-0103-6

    Figure Lengend Snippet: Effect of SAM, 25(OH)D, and their combination in vitro. a Schematic of the in vitro treatment protocol. b Human (ZR-75-1, MDA-MB-231) and murine (PyMT-R221A, E0771) breast cancer cells were treated with vehicle alone control, SAM (200 µmol·L −1 ) or 25(OH)D (100 nmol·L −1 ) alone and SAM + 25(OH)D every other day; and cell number was determined using Coulter counter on days 1,3, and 5 post-treatment. Results are shown as the mean ± SEM from at least five independent experiments. Significant differences from the control groups in each cell lines were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks. c Following treatment, 5 × 10 3 cells from the control and different treatment groups were subjected to clonogenic survival assay. The culture media was refreshed every 3–4 days for a period of about 2 weeks, the cells were then stained with crystal violet, and the total number of colonies was counted under the microscope. Results are shown as the mean ± SEM of at least five independent experiments. Significant differences were determined using ANOVA followed by post hoc Tukey’s test and are represented by asterisks

    Article Snippet: The HBEC were obtained from Celprogen (Cat# 36056-01) and supplemented with manufacturer recommended human breast epithelial cell culture serum-free media (Cat# M36056-01).

    Techniques: In Vitro, Clonogenic Cell Survival Assay, Staining, Microscopy